February 22, 2010

A group recently developed a new method for studying retinal development in chick embryos, using in ovo electroporation at embryonic day 3 or 4 (E3-E4). This method has allowed the visualization of the dynamic morphologies of differentiating retinal cells, which previously was difficult with standard injection and electroporation techniques. Image above shows GFP expression in a ganglion cell at E18, with its characteristic morphology and location within the developing retina.

Reference: Sung Tae Doh, Hailing Hao, Stephanie C Loh, Tapan Patel, Haim Y Tawil, David K Chen, Anna Pashkova, Andy Shen, Huimin Wang and Li Cai. Analysis of retinal cell development in chick embryo by immunohistochemistry and in ovo electroporation techniques.
BMC Developmental Biology 2010, 10:8. doi:10.1186/1471-213X-10-8. Article can be found here.

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