Chromosome architecture is tightly regulated during mitosis, from condensation and cohesion of chromosome arms early in mitosis, to chromosome cleavage and separation in anaphase. A recent paper uncovers a pathway that is critical for proper chromosome architecture; an ATPase called PICH works with the kinase Plk-1 to regulate chromosome arm cohesion and organization during mitosis. Image above shows chromosomes during mitosis, stained for Plk-1 (left, red in merged image), PICH (center, green in merge), and DNA (right, blue in merge).
Reference: Yasuhiro Kurasawa and Li-yuan Yu-Lee. Authors’ Molecular Biology of the Cell paper can be found here.